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Correspondence |

How Safe Could Intrathecal Transplantation of Mesenchymal Stem Cells Be Considered in Multiple Sclerosis?

Dimitris Karacostas, MD, PhD; George Hadjigeorgiou, MD, PhD; Panos Ioannidis, MD, PhD; Ioannis Milonas, MD, PhD
[+] Author Affiliations

Author Affiliations: Departments of Neurology, AHEPA University Hospital, Thessaloniki, Greece, and Thessaly University Hospital, Larissa, Greece.


Arch Neurol. 2011;68(7):955-956. doi:10.1001/archneurol.2011.161
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We read with considerable interest the exploratory study by Karussis et al1 on the safety of intrathecally transplanted mesenchymal stem cells (MSCs) in patients with multiple sclerosis (MS) and those with amyotrophic lateral sclerosis (ALS). Nevertheless, the data presented in that study1 do not clearly support the positive conclusion drawn, at least in MS.

More specifically, the scientific basis of the intrathecal route is limited because there is only 1 relevant study on the animal disease model.2 Additionally, there is, to our knowledge, no scientific evidence that the presence of MSCs within the central nervous system (CNS) is an absolute necessity for the effect these cells might have in autoimmune demyelination.

Based on magnetic resonance imaging (MRI) findings, the presence of any significant unexpected pathology up to the 6-month follow-up may not be totally excluded because the unpredicted behavior of MSCs within the CNS at the microscopic level3 might potentially be harmful, though clinically silent or undetected by a 1.5-T MRI scanner. Moreover, it is questionable whether the transplanted MSCs migrated toward MS lesions as expected2 because no relevant MRI finding was evident according to the data.1

Although, based on previous in vitro studies, the authors accept that there is a low risk of treatment-related malignant neoplasm induction, recent data indicate the spontaneous malignant transformation is biohazard in long-term ex vivo expansion of human MSCs.4

Finally, the immunomodulatory effects induced by the intravenously administered MSCs lack any evidence about T-helper 17 lymphocyte (Th17) response profiles in treated patients, thereby imposing further weakness on the concept of safety despite the profound reduction of other proinflammatory factors or the increase of regulatory T cells in patients who received transplants. In addition, recent preliminary data5 indicate that MSCs may induce Th17 responses concomitantly with Th1 response suppression. Such behavior of migrated MSCs might potentially exacerbate immune reactions within the CNS, in situ.

Within the emerging and promising field of regenerative medicine, the results and conclusions of clinical studies describing cell-based therapies should be interpreted with great caution to minimize any treatment-related harm to the already-unfortunate patients.

AUTHOR INFORMATION

Correspondence: Dr Karacostas, Department of Neurology, AHEPA University Hospital, Thessaloniki 54636, Greece (bneurol@med.auth.gr).

Financial Disclosure: None reported.

REFERENCES

Karussis D, Karageorgiou C, Vaknin-Dembinsky A,  et al.  Safety and immunological effects of mesenchymal stem cell transplantation in patients with multiple sclerosis and amyotrophic lateral sclerosis.  Arch Neurol. 2010;67(10):1187-1194
PubMed
Kassis I, Grigoriadis N, Gowda-Kurkalli B,  et al.  Neuroprotection and immunomodulation with mesenchymal stem cells in chronic experimental autoimmune encephalomyelitis.  Arch Neurol. 2008;65(6):753-761
PubMed
Hunt DP, Irvine KA, Webber DJ, Compston DA, Blakemore WF, Chandran S. Effects of direct transplantation of multipotent mesenchymal stromal/stem cells into the demyelinated spinal cord.  Cell Transplant. 2008;17(7):865-873
PubMed
Røsland GV, Svendsen A, Torsvik A,  et al.  Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.  Cancer Res. 2009;69(13):5331-5339
PubMed
Darlington PJ, Boivin MN, Renoux C,  et al.  Reciprocal Th1 and Th17 regulation by mesenchymal stem cells: implication for multiple sclerosis.  Ann Neurol. 2010;68(4):540-545
PubMed

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Karussis D, Karageorgiou C, Vaknin-Dembinsky A,  et al.  Safety and immunological effects of mesenchymal stem cell transplantation in patients with multiple sclerosis and amyotrophic lateral sclerosis.  Arch Neurol. 2010;67(10):1187-1194
PubMed
Kassis I, Grigoriadis N, Gowda-Kurkalli B,  et al.  Neuroprotection and immunomodulation with mesenchymal stem cells in chronic experimental autoimmune encephalomyelitis.  Arch Neurol. 2008;65(6):753-761
PubMed
Hunt DP, Irvine KA, Webber DJ, Compston DA, Blakemore WF, Chandran S. Effects of direct transplantation of multipotent mesenchymal stromal/stem cells into the demyelinated spinal cord.  Cell Transplant. 2008;17(7):865-873
PubMed
Røsland GV, Svendsen A, Torsvik A,  et al.  Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.  Cancer Res. 2009;69(13):5331-5339
PubMed
Darlington PJ, Boivin MN, Renoux C,  et al.  Reciprocal Th1 and Th17 regulation by mesenchymal stem cells: implication for multiple sclerosis.  Ann Neurol. 2010;68(4):540-545
PubMed

Correspondence

July 1, 2011
Dimitrios Karussis, MD, PhD; Adi Vaknin-Debminsky, MD, PhD; Oded Abramsky, MD, PhD; Ibrahim Kassis, PhD; Panayiota Petrou, MD; Tamir Ben Hur, MD, PhD
Arch Neurol. 2011;68(7):955-956. doi:10.1001/archneurol.2011.162.
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