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Fibroblasts in Huntington's Disease

Ara Y. Tourian, MD; Wu-Yen Hung, MD
Arch Neurol. 1984;41(3):251-251. doi:10.1001/archneur.1984.04050150029007
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To the Editor.  —I read with interest the article by Archer and Mancall1 in the Archives. The authors claim that their results are a duplication and extension of cell attachment and hexosamine studies reported by us.2-4 In fact, they merely subjected our hypothesis to statistical analysis. The view that the only variable in their study was the number of cell lines is misleading and incorrect. A wide range of variables were introduced by the authors, including those in procedure, type, and use of fetal calf serum; the plastic surface to which the cells were attached; and the use of maintenance (nongrowing) cell populations to test cell attachment and nutritional response.The authors claim to have tested 20 cell lines from each genotype for their results. However, only five cell lines were used for attachment studies (their Fig 2), and eight and six cell lines were used for growth

REFERENCES

Archer FJ, Mancall LE:  Cultured fibroblasts in Huntington's disease: I. Effects of L-glutamic acid . Arch Neurol 1983;;40:19-23.
Tourian A, Hung WY:  Glucosamine dependence of Huntington's chorea fibroblasts in culture . Biochem Biophys Res Commun 1977;;76:345-353.
Tourian A, Hung WY:  Membrane abnormalities of Huntington's chorea fibroblasts in culture . Biochem Biophys Res Commun 1977;; 78:1296-1303.
Tourian A, Hung WY:  Huntington's disease fibroblasts: Nutritional and protein glycosylation studies , in Chase TN, Wexler NS, Barbeau A (eds): Advances in Neurology . New York, Raven Press, 1979;, vol 23, pp 371-386.
Hung WY, Mold DE, Tourian A:  Huntington's chorea fibroblasts: Cellular protein glycosylation . Biochem J 1980;;190:711-719.
Hung WY, Tourian A:  Differential labelling of UDP-N-acetylglucosamine in Huntington's chorea fibroblasts . Biochem J 1981;;196:495-498.
Hung WY, Tourian A: The impaired conversion of (14C) glucose to (14C) UDP-hexosamines. Program and abstracts of the Annual Meeting of the Society for the Study of Inborn Errors of Metabolism, Southhampton, England, 1981.
Cassiman JJ, Verlinden J, Vlientick RF, et al:  Qualitative and quantitative study of the growth and cell surface properties of Huntington's disease fibroblasts and age matched controls: Lack of significant differences . Hum Genet 1979;;53:75-86.

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Archer FJ, Mancall LE:  Cultured fibroblasts in Huntington's disease: I. Effects of L-glutamic acid . Arch Neurol 1983;;40:19-23.
Tourian A, Hung WY:  Glucosamine dependence of Huntington's chorea fibroblasts in culture . Biochem Biophys Res Commun 1977;;76:345-353.
Tourian A, Hung WY:  Membrane abnormalities of Huntington's chorea fibroblasts in culture . Biochem Biophys Res Commun 1977;; 78:1296-1303.
Tourian A, Hung WY:  Huntington's disease fibroblasts: Nutritional and protein glycosylation studies , in Chase TN, Wexler NS, Barbeau A (eds): Advances in Neurology . New York, Raven Press, 1979;, vol 23, pp 371-386.
Hung WY, Mold DE, Tourian A:  Huntington's chorea fibroblasts: Cellular protein glycosylation . Biochem J 1980;;190:711-719.
Hung WY, Tourian A:  Differential labelling of UDP-N-acetylglucosamine in Huntington's chorea fibroblasts . Biochem J 1981;;196:495-498.
Hung WY, Tourian A: The impaired conversion of (14C) glucose to (14C) UDP-hexosamines. Program and abstracts of the Annual Meeting of the Society for the Study of Inborn Errors of Metabolism, Southhampton, England, 1981.
Cassiman JJ, Verlinden J, Vlientick RF, et al:  Qualitative and quantitative study of the growth and cell surface properties of Huntington's disease fibroblasts and age matched controls: Lack of significant differences . Hum Genet 1979;;53:75-86.

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