Error in Figure in: Episodic Ataxia Associated With EAAT1 Mutation C186S Affecting Glutamate Reuptake

EAAT1 C186S mutation. A, Schematic representation of the EAAT1 protein and the location of the mutated Cys¹⁸⁶ amino acid in transmembrane segment 4B (indicated by a black dot) (the structure is adapted from Yernool et al¹⁴). B, Conservation of the mutated residue Cys¹⁸⁶ highlighted in gray. The protein sequences were obtained from GenBank (homo sapiens, NP_004163; Bos taurus, NP_683740; Mus musculus, NP_683740; Rattus norvegicus, NP_062098; salamander, O57321; Danio rerio, NP_997805; Drosophila melanogaster, NP_477428; human EAAT2, AY066021; human EAAT3, NP_004161; human EAAT4, NP_005062; human EAAT5, NP_006662). C, Glutamate uptake assay in COS7 cells expressing mutant EAAT1-186S (mean [SEM], 88.2 [5.5]) or wild-type EAAT1-186C (mean [SEM], 107.8 [6.9]). The results are the mean (SEM) of the 4 experiments, each in triplicate. The values are picomoles of glutamate transported per milligram of protein per minute of incubation. Asterisk indicates significant reduction of glutamate uptake compared with wild type (P = .029). Error bars indicate SEM. HP indicates helical hairpin.