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Juvenile Alpers Disease FREE

Esko Wiltshire, MD, FRACP; Guido Davidzon, MD; Salvatore DiMauro, MD; Hasan O. Akman, PhD; Lynette Sadleir, MD, FRACP; Lindsay Haas, MBChB, FRACP; Jane Zuccollo, MBBChBAO, FRCPA; Alison McEwen, BA, FHGSA; David R. Thorburn, PhD
[+] Author Affiliations

Author Affiliations: Departments of Pediatrics and Child Health (Drs Wiltshire and Sadleir) and Pathology (Dr Zuccollo), Wellington School of Medicine and Health Sciences, University of Otago, and Central Regional Genetics Service (Dr Wiltshire and Ms McEwen) and Department of Neurology (Ms Haas), Capital and Coast District Health Board, Wellington, New Zealand; Department of Neurology, Columbia University Medical Center, New York, New York (Drs Davidzon, DiMauro, and Akman); and Murdoch Childrens Research Institute and Genetic Health Services Victoria, Royal Children's Hospital, and Department of Paediatrics, University of Melbourne, Melbourne, Australia (Dr Thorburn).


Arch Neurol. 2008;65(1):121-124. doi:10.1001/archneurol.2007.14.
Text Size: A A A
Published online

ABSTRACT

Background  Alpers disease is commonly associated with polymerase γ deficiency and usually affects infants or young children.

Objective  To report a juvenile case of Alpers disease due to mutations in the polymerase γ gene (POLG1).

Design  Clinical, pathologic, biochemical, and molecular analysis.

Setting  Tertiary care university hospital and academic institutions.

Patient  A 17-year-old adolescent girl with intractable epilepsy and liver disease.

Main Outcome Measures  Clinical course and pathologic, biochemical, and molecular features.

Results  Biochemical and pathologic evidence suggested a respiratory chain defect, which was confirmed by enzyme analysis of the liver. Mutational analysis of POLG1 showed 2 novel mutations: T851A and R1047W.

Conclusion  The POLG1 mutations can cause juvenile and childhood Alpers disease.

Figures in this Article

Alpers disease, or progressive neuronal degeneration of childhood (Online Mendelian Inheritance in Man [OMIM] 203700), is characterized by developmental regression, intractable epilepsy, progressive neurologic deterioration, liver disease, and death usually before 10 years of age.13 Neuropathologic changes include patchy neuronal loss and gliosis, particularly in the striate cortex,4 whereas the liver shows steatosis, cellular necrosis, focal inflammation, and fibrosis.5 In 2004, Alpers disease was attributed to mutations in the catalytic subunit of the mitochondrial DNA (mtDNA) polymerase gene polymerase γ (POLG1),6 and this association has been confirmed in several series.710

Alpers disease is typically a disease of childhood, and it may not be given sufficient attention in juvenile cases. We describe a 17-year-old adolescent girl with clinical, electroencephalographic, pathologic, and biochemical features of Alpers disease caused by mutations in POLG1 and suggest that this diagnosis be considered in adults with encephalopathy or intractable epilepsy, especially with liver disease. In these patients, mutational analysis of POLG1 is mandatory.

REPORT OF A CASE

A 17-year-old adolescent girl of New Zealand, European, and Pacific Island ethnicity was initially seen with clusters of occipital seizures characterized by a brief visual disturbance followed by head extension, clonic jerking of the right arm, and secondary generalization. An initial electroencephalogram demonstrated diffuse slowing. Handwriting had always been difficult, to the extent that she needed a stenographer to write some final examinations. Mild clumsiness and pes cavus had worsened during the previous 2 years. Nerve conduction studies showed peripheral neuropathy. Developmental milestones were otherwise normal, and she had normal intelligence. Her vision and hearing were also normal. Pregnancy and delivery had been unremarkable, and her height and weight were consistently between the third and tenth percentiles. At age 5 years, she had viral meningitis (coxsackievirus B2 cultured from cerebrospinal fluid). The cerebrospinal fluid at this stage had a low glucose level (25 mg/dL [to convert to millimoles per liter, multiply by 0.0555]) and a significantly elevated protein level (0.57 g/dL [to convert to grams per liter, multiply by 10.0]), out of keeping with viral meningitis. She made a full recovery. She began experiencing migraines at age 12 years, with visual aura and vomiting.

Family history showed that a maternal aunt, currently aged 48 years, had an undiagnosed neurologic condition, with delayed early milestones and ataxia beginning at age 3 years. She regressed neurologically between ages 5 and 7 years, with subsequent severe intellectual disability. A maternal uncle died at age 3 months of “cot death.”

The patient was treated early with midazolam hydrochloride followed by maintenance therapy with phenytoin sodium. She had a cluster of seizures after 1 month, remained seizure-free for 4 months, and then developed status epilepticus. During the next 2 months, she had repeated admissions to the intensive care unit with status epilepticus or encephalopathy. Although she had lucid periods between episodes, overall she developed stepwise deterioration, with memory impairment, slurred speech, and left-sided hemiparesis. A variety of anticonvulsant agents, including carbamazepine, valproate sodium, lamotrigine, intravenous benzodiazepines, topiramate, gabapentin, and intermittent doses of phenobarbital sodium, were tried without sustained benefit. Liver function test results became abnormal while the patient was taking carbamazepine and before valproate therapy: peak alanine aminotransferase, 396 U/L (reference range, 7-28 U/L) (to convert to microkatals per liter, multiply by 0.0167); peak γ-glutamyltransferase, 1234 U/L (reference range, 7-36 U/L) (to convert to microkatals per liter, multiply by 0.0167); and peak aspartate aminotransferase, 322 U/L (reference range, 12-27 U/L) (to convert to microkatals per liter, multiply by 0.0167). Liver dysfunction persisted when valproate therapy was commenced but deteriorated when the patient received multiple drugs, including antituberculous therapy. Subsequent electroencephalograms showed frequent epileptiform discharges in the left posterior quadrant and continued diffuse slowing. Magnetic resonance imaging findings were initially normal but showed progressive abnormality with increased signal on T2-weighted images in the cortical and subcortical white matter and basal ganglia (Figure 1). The cerebrospinal fluid showed persistently low glucose levels (11-65 mg/dL) and elevated protein levels (0.11-0.47 g/dL), with few lymphocytes. Lactic acidosis (lactate levels of 61-110 mg/dL [to convert to millimoles per liter, multiply by 0.111]) was noted during the 2 weeks before death. Urinalysis showed generalized aminoaciduria and normal organic acid levels. Biopsies of the skin, muscle, and liver were nondiagnostic. Brain biopsy showed slight perivascular lymphocytic cuffing, perhaps representing cerebral angiitis.

Place holder to copy figure label and caption
Figure 1.

Axial T2-weighted magnetic resonance image showing an increased cortical signal, particularly in the occipital lobes.

Graphic Jump Location

A variety of infectious, inflammatory, and neoplastic processes were considered. The patient received empirical therapy for these conditions (immunosuppression, antibiotics, and therapy for tuberculosis), without any improvement. Consultation with a pediatric neurologist suggested the possibility of a mitochondrial disorder. She commenced a high-fat, low-carbohydrate diet and multivitamin therapy, with no improvement. Her neurologic decline continued, and she died at age 17 years 9 months of respiratory failure secondary to her neurologic condition. At autopsy, the brain showed extensive neuronal loss and gliosis, most prominent in the occipital lobes (Figure 2B), as typically seen in Alpers disease, but also in the basal ganglia and brainstem. The liver showed extensive steatosis and fresh necrosis (Figure 2A).

Place holder to copy figure label and caption
Figure 2.

Histopathologic features of the brain and liver. A, Liver histologic examination (hematoxylin-eosin, original magnification ×40) showing microvesicular steatosis with central nuclei. B, Glial fibrillary acid protein stain (original magnification ×40) of the patient's cerebral cortex showing activated astrocytes, a sign of gliosis. C, Glial fibrillary acid protein stain (original magnification ×40) of the normal cerebral cortex.

Graphic Jump Location

Respiratory chain enzyme analysis and real-time quantitative polymerase chain reaction for estimation of mtDNA content, performed on a muscle biopsy sample as described previously,8,11 showed normal activities of complexes I, II, II+III, III, and IV and of mitochondrial marker enzyme citrate synthase. In the liver (post mortem), there was marked deficiency of the respiratory chain enzymes containing subunits encoded by mtDNA (residual activities of 8% for complex I, 33% for complex III, and 19% for complex IV), with normal activity of the nuclear-encoded complex II (101%) and elevated activity of citrate synthase (316%).

Sequencing of the entire mitochondrial genome in the liver DNA did not reveal any pathogenic mutations. Quantitative polymerase chain reaction showed that the ratio of mtDNA to nuclear DNA in the liver was deficient (0.17; mean [SD] of 6 control livers, 1.01 [0.15]; range, 0.78-1.19).

The POLG1 gene was screened by direct sequencing as described previously8 and was found to harbor 2 compound heterozygous missense mutations. The first mutation, A2551G in exon 16, predicts the substitution of a conserved threonine by an alanine at position 851 (T851A) (Figure 3A). The second mutation, C3139T in exon 20, changes a conserved arginine to a tryptophan at position 1047 (R1047W) (Figure 3B). These mutations are assumed to be pathogenic because they are not reported polymorphic changes, are absent from more than 200 control alleles, and change highly conserved amino acids. The threonine residue at codon 851 is in a region that is conserved in mice, frogs, flies, and yeast and that includes another pathogenic POLG1 mutation, G848S.12 The R1047W mutation changes the same arginine residue mutated by another previously reported pathogenic mutation, R1047Q.13 The patient's maternal aunt does not carry either mutation.

Place holder to copy figure label and caption
Figure 3.

Electropherogram of the polymerase γ gene sequence showing the 2 novel mutations found in this compound heterozygous patient: A2551G in exon 16, resulting in a T851A amino acid change (A), and C3139T in exon 20, resulting in an R1047W amino acid change (B).

Graphic Jump Location

COMMENT

Alpers disease is characterized by childhood encephalopathy and hepatopathy due to mitochondrial respiratory chain deficiency. Some adults with Alpers disease have been described but have not been studied at the molecular level.14,15 Mutations in POLG1 were first identified as a cause of Alpers disease by Naviaux and Nguyen,6 and recent studies7,8,16 have confirmed this association, showing that deficiency of polymerase γ is the most common autosomal recessive cause of Alpers disease in children. The present data suggest that mutations in POLG1 are also involved in juvenile Alpers disease.

The pattern of respiratory chain abnormality in the proband (complex I and IV deficiency) and the family history of a maternal aunt with neurologic regression and ataxia led to the initial consideration of mtDNA mutations. However, sequencing of the whole mitochondrial genome excluded this possibility. Finding mutations in POLG1 enabled us to also test the aunt, who carries neither mutation, indicating that her neurologic problems are unrelated. Confirmation of autosomal recessive (rather than maternal) inheritance has facilitated genetic counseling.

Mutations in POLG1 were originally associated with autosomal dominant or recessive familial progressive external ophthalmoplegia12,17 and multiple mtDNA rearrangements (particularly deletions) in postmitotic tissues. In addition, mutations in POLG1 can cause sensory ataxic neuropathy with dysarthria and ophthalmoplegia18 (of note, the present patient also had a peripheral neuropathy) and an ataxic syndrome without progressive external ophthalmoplegia.19 Alpers disease is associated with depletion of mtDNA in the liver.8,20 Thus, mutations in this gene can have various consequences for mtDNA and variously affect different tissues.21

Metabolic diseases are less likely in adults than in children. The present patient, however, had several features that made a mitochondrial disorder more likely than acquired conditions, including symptoms and signs reflecting the involvement of multiple systems (brain, peripheral nervous system, liver, and kidney, as well as lactic acidosis). Mitochondrial disorders should be considered at any age, particularly when multiple organ systems are involved.

In conclusion, Alpers disease should be considered in adults with encephalopathy and intractable epilepsy, particularly when the liver or other organs are involved. Mutation screening of POLG1 should be considered in such patients and is vital for identifying the underlying cause and for genetic counseling.

ARTICLE INFORMATION

Correspondence: Salvatore DiMauro, MD, 3-313 Russ Berrie Medical Science Pavilion, 1150 St Nicholas Ave, New York, NY 10032 (sd12@columbia.edu).

Accepted for Publication: March 9, 2007.

Author Contributions: Dr DiMauro had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Wiltshire, Sadleir, and Thorburn. Acquisition of data: Wiltshire, Davidzon, Akman, Sadleir, Haas, Zuccollo, McEwen, and Thorburn. Analysis and interpretation of data: Wiltshire, Davidzon, DiMauro, Akman, Zuccollo, and Thorburn. Drafting of the manuscript: Wiltshire, Akman, and Sadleir. Critical revision of the manuscript for important intellectual content: Wiltshire, Davidzon, DiMauro, Sadleir, Haas, Zuccollo, McEwen, and Thorburn. Obtained funding: DiMauro and Thorburn. Administrative, technical, and material support: Wiltshire, Davidzon, Akman, Sadleir, and Thorburn. Study supervision: DiMauro.

Financial Disclosure: None reported.

Funding/Support: This study was supported by grants NS11766 and HD32062 from the National Institutes of Health, by grants from the Muscular Dystrophy Association and the Australian National Health and Medical Research Council, and by the Marriott Mitochondrial Disorder Clinical Research Fund.

Additional Contributions: Robert W. Taylor, PhD, and Douglass M. Turnbull, FRCP, University of Newcastle upon Tyne, performed the mtDNA sequencing.

REFERENCES

Alpers  BJ Diffuse progressive degeneration of the gray matter of the cerebrum. Arch Neurol Psychiatry 1931;25469- 505
Link to Article
Huttenlocher  PRSolitaire  GBAdams  G Infantile diffuse cerebral degeneration with hepatic cirrhosis. Arch Neurol 1976;33 (3) 186- 192
PubMed Link to Article
Harding  BN Progressive neuronal degeneration of childhood with liver disease (Alpers-Huttenlocher syndrome): a personal review. J Child Neurol 1990;5 (4) 273- 287
PubMed Link to Article
Harding  BNEgger  JPortmann  BErdohazi  M Progressive neuronal degeneration of childhood with liver disease: a pathological study. Brain 1986;109181- 206
PubMed Link to Article
Narkewicz  MRSokol  RJBeckwith  B  et al.  Liver involvement in Alpers disease. J Pediatr 1991;119 (2) 260- 267
PubMed Link to Article
Naviaux  RKNguyen  KV POLG mutations associated with Alpers' syndrome and mitochondrial DNA depletion. Ann Neurol 2004;55 (5) 706- 712
PubMed Link to Article
Ferrari  GLamantea  EDonati  A  et al.  Infantile hepatocerebral syndromes associated with mutations in the mitochondrial DNA polymerase-γA. Brain 2005;128 (pt 4) 723- 731
PubMed Link to Article
Davidzon  GMancuso  MFerraris  S  et al.  POLG mutations and Alpers syndrome. Ann Neurol 2005;57 (6) 921- 924
PubMed Link to Article
Horvath  RHudson  GFerrari  G  et al.  Phenotypic spectrum associated with mutations of the mitochondrial polymerase γ gene. Brain 2006;129 (pt 7) 1674- 1684
PubMed Link to Article
Tzoulis  CEngelsen  BATelstad  W  et al.  The spectrum of clinical disease caused by the A467T and W748S POLG mutations: a study of 26 cases. Brain 2006;129 (pt 7) 1685- 1692
PubMed Link to Article
Kirby  DMCrawford  MCleary  MA  et al.  Respiratory chain complex I deficiency: an underdiagnosed energy generation disorder. Neurology 1999;52 (6) 1255- 1264
PubMed Link to Article
Lamantea  ETiranti  VBordoni  A  et al.  Mutations of mitochondrial DNA polymerase γA are a frequent cause of autosomal dominant or recessive progressive external ophthalmoplegia. Ann Neurol 2002;52 (2) 211- 219
PubMed Link to Article
Agostino  AValletta  LChinnery  PF  et al.  Mutations of ANT1, Twinkle, and POLG1 in sporadic progressive external ophthalmoplegia (PEO). Neurology 2003;60 (8) 1354- 1356
PubMed Link to Article
Wörle  HKohler  BSchlote  W  et al.  Progressive cerebral degeneration of childhood with liver disease (Alpers Huttenlocher disease) with cytochrome oxidase deficiency presenting with epilepsia partialis continua as the first clinical manifestation. Clin Neuropathol 1998;17 (2) 63- 68
PubMed
Harding  BNAlsanjari  NSmith  SJM  et al.  Progressive neuronal degeneration of childhood with liver disease (Alpers' disease) presenting in young adults. J Neurol Neurosurg Psychiatry 1995;58 (3) 320- 325
PubMed Link to Article
Nguyen  KVOstergard  ERavn  SH  et al.  POLG mutations in Alpers syndrome. Neurology 2005;65 (9) 1493- 1495
PubMed Link to Article
Van Goethem  GDermaut  BLofgren  A  et al.  Mutation of POLG is associated with progressive external ophthalmoplegia characterized by mtDNA deletions. Nat Genet 2001;28 (3) 211- 212
PubMed Link to Article
Van Goethem  GMartin  JJDermaut  B  et al.  Recessive POLG mutations presenting with sensory and ataxic neuropathy in compound heterozygote patients with progressive external ophthalmoplegia. Neuromuscul Disord 2003;13 (2) 133- 142
PubMed Link to Article
Van Goethem  GLuoma  PRantamaki  M  et al.  POLG mutations in neurodegenerative disorders with ataxia but no muscle involvement. Neurology 2004;63 (7) 1251- 1257
PubMed Link to Article
Naviaux  RKNyhan  WLBarshop  BA  et al.  Mitochondrial DNA polymerase gamma deficiency and mtDNA depletion in a child with Alpers' syndrome. Ann Neurol 1999;45 (1) 54- 58
PubMed Link to Article
DiMauro  SDavidzon  GHirano  M A polymorphic polymerase. Brain 2006;129 (pt 7) 1637- 1639
PubMed Link to Article

Figures

Place holder to copy figure label and caption
Figure 1.

Axial T2-weighted magnetic resonance image showing an increased cortical signal, particularly in the occipital lobes.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

Histopathologic features of the brain and liver. A, Liver histologic examination (hematoxylin-eosin, original magnification ×40) showing microvesicular steatosis with central nuclei. B, Glial fibrillary acid protein stain (original magnification ×40) of the patient's cerebral cortex showing activated astrocytes, a sign of gliosis. C, Glial fibrillary acid protein stain (original magnification ×40) of the normal cerebral cortex.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.

Electropherogram of the polymerase γ gene sequence showing the 2 novel mutations found in this compound heterozygous patient: A2551G in exon 16, resulting in a T851A amino acid change (A), and C3139T in exon 20, resulting in an R1047W amino acid change (B).

Graphic Jump Location

Tables

References

Alpers  BJ Diffuse progressive degeneration of the gray matter of the cerebrum. Arch Neurol Psychiatry 1931;25469- 505
Link to Article
Huttenlocher  PRSolitaire  GBAdams  G Infantile diffuse cerebral degeneration with hepatic cirrhosis. Arch Neurol 1976;33 (3) 186- 192
PubMed Link to Article
Harding  BN Progressive neuronal degeneration of childhood with liver disease (Alpers-Huttenlocher syndrome): a personal review. J Child Neurol 1990;5 (4) 273- 287
PubMed Link to Article
Harding  BNEgger  JPortmann  BErdohazi  M Progressive neuronal degeneration of childhood with liver disease: a pathological study. Brain 1986;109181- 206
PubMed Link to Article
Narkewicz  MRSokol  RJBeckwith  B  et al.  Liver involvement in Alpers disease. J Pediatr 1991;119 (2) 260- 267
PubMed Link to Article
Naviaux  RKNguyen  KV POLG mutations associated with Alpers' syndrome and mitochondrial DNA depletion. Ann Neurol 2004;55 (5) 706- 712
PubMed Link to Article
Ferrari  GLamantea  EDonati  A  et al.  Infantile hepatocerebral syndromes associated with mutations in the mitochondrial DNA polymerase-γA. Brain 2005;128 (pt 4) 723- 731
PubMed Link to Article
Davidzon  GMancuso  MFerraris  S  et al.  POLG mutations and Alpers syndrome. Ann Neurol 2005;57 (6) 921- 924
PubMed Link to Article
Horvath  RHudson  GFerrari  G  et al.  Phenotypic spectrum associated with mutations of the mitochondrial polymerase γ gene. Brain 2006;129 (pt 7) 1674- 1684
PubMed Link to Article
Tzoulis  CEngelsen  BATelstad  W  et al.  The spectrum of clinical disease caused by the A467T and W748S POLG mutations: a study of 26 cases. Brain 2006;129 (pt 7) 1685- 1692
PubMed Link to Article
Kirby  DMCrawford  MCleary  MA  et al.  Respiratory chain complex I deficiency: an underdiagnosed energy generation disorder. Neurology 1999;52 (6) 1255- 1264
PubMed Link to Article
Lamantea  ETiranti  VBordoni  A  et al.  Mutations of mitochondrial DNA polymerase γA are a frequent cause of autosomal dominant or recessive progressive external ophthalmoplegia. Ann Neurol 2002;52 (2) 211- 219
PubMed Link to Article
Agostino  AValletta  LChinnery  PF  et al.  Mutations of ANT1, Twinkle, and POLG1 in sporadic progressive external ophthalmoplegia (PEO). Neurology 2003;60 (8) 1354- 1356
PubMed Link to Article
Wörle  HKohler  BSchlote  W  et al.  Progressive cerebral degeneration of childhood with liver disease (Alpers Huttenlocher disease) with cytochrome oxidase deficiency presenting with epilepsia partialis continua as the first clinical manifestation. Clin Neuropathol 1998;17 (2) 63- 68
PubMed
Harding  BNAlsanjari  NSmith  SJM  et al.  Progressive neuronal degeneration of childhood with liver disease (Alpers' disease) presenting in young adults. J Neurol Neurosurg Psychiatry 1995;58 (3) 320- 325
PubMed Link to Article
Nguyen  KVOstergard  ERavn  SH  et al.  POLG mutations in Alpers syndrome. Neurology 2005;65 (9) 1493- 1495
PubMed Link to Article
Van Goethem  GDermaut  BLofgren  A  et al.  Mutation of POLG is associated with progressive external ophthalmoplegia characterized by mtDNA deletions. Nat Genet 2001;28 (3) 211- 212
PubMed Link to Article
Van Goethem  GMartin  JJDermaut  B  et al.  Recessive POLG mutations presenting with sensory and ataxic neuropathy in compound heterozygote patients with progressive external ophthalmoplegia. Neuromuscul Disord 2003;13 (2) 133- 142
PubMed Link to Article
Van Goethem  GLuoma  PRantamaki  M  et al.  POLG mutations in neurodegenerative disorders with ataxia but no muscle involvement. Neurology 2004;63 (7) 1251- 1257
PubMed Link to Article
Naviaux  RKNyhan  WLBarshop  BA  et al.  Mitochondrial DNA polymerase gamma deficiency and mtDNA depletion in a child with Alpers' syndrome. Ann Neurol 1999;45 (1) 54- 58
PubMed Link to Article
DiMauro  SDavidzon  GHirano  M A polymorphic polymerase. Brain 2006;129 (pt 7) 1637- 1639
PubMed Link to Article

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