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Original Investigation |

Elevated Serum Pesticide Levels and Risk for Alzheimer Disease FREE

Jason R. Richardson, PhD1,2; Ananya Roy, ScD2; Stuart L. Shalat, ScD1,2; Richard T. von Stein, PhD2; Muhammad M. Hossain, PhD1,2; Brian Buckley, PhD2; Marla Gearing, PhD4; Allan I. Levey, MD, PhD3; Dwight C. German, PhD5
[+] Author Affiliations
1Department of Environmental and Occupational Medicine, Rutgers Robert Wood Johnson Medical School, Piscataway, New Jersey
2Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey
3Department of Neurology and Alzheimer’s Disease, Emory University School of Medicine, Atlanta, Georgia
4Department of Pathology, Emory University School of Medicine, Atlanta, Georgia
5Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, Texas
JAMA Neurol. 2014;71(3):284-290. doi:10.1001/jamaneurol.2013.6030.
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Published online

Importance  The causes of late-onset Alzheimer disease (AD) are not yet understood but likely include a combination of genetic, environmental, and lifestyle factors. Limited epidemiological studies suggest that occupational pesticide exposures are associated with AD. Previously, we reported that serum levels of dichlorodiphenyldichloroethylene (DDE), the metabolite of the pesticide dichlorodiphenyltrichloroethane (DDT), were elevated in a small number of patients with AD (n=20).

Objective  To evaluate the association between serum levels of DDE and AD and whether the apolipoprotein E (APOE) genotype modifies the association.

Design, Setting, and Participants  A case-control study consisting of existing samples from patients with AD and control participants from the Emory University Alzheimer’s Disease Research Center and the University of Texas Southwestern Medical School’s Alzheimer’s Disease Center. Serum levels of DDE were measured in 79 control and 86 AD cases.

Main Outcomes and Measures  Serum DDE levels, AD diagnosis, severity of AD measured by the Mini-Mental State Examination score, and interaction with APOE4 status.

Results  Levels of DDE were 3.8-fold higher in the serum of those with AD (mean [SEM], 2.64 [0.35] ng/mg cholesterol) when compared with control participants (mean [SEM], 0.69 [0.1] ng/mg cholesterol; P < .001). The highest tertile of DDE levels was associated with an odds ratio of 4.18 for increased risk for AD (95% CI, 2.54-5.82; P < .001) and lower Mini-Mental State Examination scores (−1.605; range, −3.095 to −0.114; P < .0001). The Mini-Mental State Examination scores in the highest tertile of DDE were −1.753 points lower in the subpopulation carrying an APOE ε4 allele compared with those carrying an APOE ε3 allele (P interaction = .04). Serum levels of DDE were highly correlated with brain levels of DDE (ρ = 0.95). Exposure of human neuroblastoma cells to DDT or DDE increased levels of amyloid precursor protein.

Conclusions and Relevance  Elevated serum DDE levels are associated with an increased risk for AD and carriers of an APOE4 ε4 allele may be more susceptible to the effects of DDE. Both DDT and DDE increase amyloid precursor protein levels, providing mechanistic plausibility for the association of DDE exposure with AD. Identifying people who have elevated levels of DDE and carry an APOE ε4 allele may lead to early identification of some cases of AD.

Figures in this Article

Alzheimer disease (AD) is the most common neurodegenerative disease worldwide and cases are expected to increase 3-fold over the next 40 years.1 The most common form of AD is the late-onset form, which typically develops after 60 years of age. The etiological factors of late-onset AD are not yet completely understood but include genetic, environmental, and lifestyle factors that influence a person’s risk for developing the disease.2 Although there is a growing list of AD susceptibility genes, only having an apolipoprotein E4 (APOE4) allele has a relatively strong effect (relative risk approximately 2-3), and, cumulatively, the more than 10 genes identified thus far account for only less than half of AD cases.3 To our knowledge, few studies have explored the potential of environmental exposures to contribute to AD, but occupational exposure to metals, solvents, and pesticide is reported to be a potential environmental contributor.4,5 Previously, we reported that serum levels of p,p’-dichlorodiphenyldichloroethylene (DDE), a metabolite of the organochlorine pesticide dichlorodiphenyltrichloroethane (DDT), were significantly higher in a small cohort (n = 20) of patients with AD compared with control participants, and that there was a significant association between DDE levels and a diagnosis of AD.6 In the present study, we evaluated the associations between serum DDE levels, AD, and Mini-Mental State Examination (MMSE) scores in a larger number of cases and control participants from 2 geographical sites, and we explored differential susceptibility by APOE4 genotype status. We also examined the relationship between brain and serum levels of DDE and whether DDT or DDE alters the expression of the amyloid precursor protein (APP) in cultured neuronal cells.

Study Population

Existing serum samples were obtained from control participants and patients with AD who were evaluated in the Alzheimer’s Disease Research Centers at the University of Texas Southwestern Medical Center (UTSW) and Emory University between 2002 and 2008. Participants who provided samples were diagnosed and assigned to AD or normal control groups based on consensus diagnosis. Normal control participants were determined to have normal neurological/clinical examinations and neuropsychological functioning findings on standardized testing. The inclusion criteria were as follows: (1) MMSE score of 28 to 30 for the control participants, (2) no structural brain abnormalities indicated by magnetic resonance imaging; and/or (3) normal general neurological examination; and (4) normal Consortium to Establish a Registry for Alzheimer’s Disease battery results. Patients with AD were diagnosed by applying National Institute of Neurological and Communicative Disorders–Alzheimer’s Disease and Related Disorders Association criteria for probable AD based on neurological and neuropsychological examination results, brain imaging, and laboratory assessments to rule out other causes of dementia at both UTSW and Emory University. Blood samples for serum preparation and genotyping were generally taken at enrollment along with MMSE. All participants had APOE genotype determined by standard TaqMan polymerase chain reaction.

Data from 43 control samples and 20 AD samples from UTSW reported in our first study were included in this analysis.6 An additional 11 control and 41 AD samples were provided by UTSW, and 25 control and 25 AD samples were provided by Emory University. Serum samples were randomly selected from existing samples collected between 2002 and 2008, and an attempt was made to match these based on age, sex, and race/ethnicity.

Matched brain and serum samples of patients with AD were obtained from the Alzheimer’s Disease Center at Washington University to determine whether serum levels of DDE correlated with brain levels. All samples (n = 11; average age = 85.7 years) were from patients diagnosed as having AD by National Institute of Neurological and Communicative Disorders–Alzheimer’s Disease and Related Disorders Association criteria and verified histopathologically following death. Blood samples were taken an average of 193 days before death and brain samples were obtained after an average postmortem interval of 12 hours.

The institutional review boards of UTSW, Washington University, Emory University, and the Robert Wood Johnson Medical School approved all of the protocols and procedures. All participants reviewed and signed written approved informed consent documents.

Assessment of Pesticide Levels

Serum DDE levels were determined by gas chromatography/mass spectrometry, as described previously,6,7 and expressed in terms of free cholesterol levels. The limit of detection for DDE was approximately 100 pg/mL. For brain pesticide determination, approximately 150 mg of temporal cortex was sonicated in a 1:1 mixture of acetone and hexane containing 5 µL of an internal standard (4-4’-DDT 13C, 1 mg/mL). Following an overnight incubation, the sample was centrifuged for 10 minutes at 3500 rpm and supernatant removed. The extraction procedure was repeated 4 times and the extract reduced by evaporation under nitrogen. The dried residue was reconstituted in acetone:hexane and applied to a solid-phase extraction column containing 5 g of Florisil and 1.5 g of anhydrous sodium sulfate preconditioned with 8 mL of hexane. Analytes were eluted with methyl tert-butyl ether, evaporated to dryness, and reconstituted in 1.8 mL of hexane for GC/MS analysis, as described previously.6,7

In Vitro Exposure to DDT/DDE and Analysis of APP Levels

SH-SY5Y cells (ATCC) were differentiated by reducing serum concentration to 1% and the addition of 1 μM retinoic acid to culture media. Cells were then exposed to DDE or DDT for 48 hours, washed with phosphate-buffered saline, and fixed in 4% paraformaldehyde. Cells were incubated with anti-APP (Sigma Aldrich) and MAP2 (Millipore) primary antibodies, followed by species-appropriate fluorescently labeled secondary antibodies (Jackson Laboratories). Images were captured on a Zeiss Observer D1 microscope (Zeiss Inc) with an X-Cite series 120Q fluorescent illuminator and a Jenoptik camera with ProgRes CapturePro 2.8 software (Jenoptik). Optical density per intensity of fluorescence against APP stain was quantified in individual cells using Image-Pro Plus 7.0 software (Media Cybernetics Inc). Data were calculated as mean (SEM) density/intensity from 3 individual experiments, each performed in triplicate, and data calculated as the percentage of control.

Statistical Analyses

All analyses were conducted with SAS software version 9 (SAS Institute Inc) or Stata version 12. We used nonparametric analysis of variance (Kruskal-Wallis) for bivariate analysis to explore the association between DDE, AD, and other covariates. Correlations between serum and brain levels of DDE were examined using the Pearson correlation coefficient.

Unconditional logistic regression, controlling for age, sex, and location, was used to estimate odds ratios (ORs) and their 95% CIs for the association between serum DDE levels and AD diagnosis in the UTSW and Emory study locations. Generalized estimating equations were used to determine the odds of having AD or decrease in MMSE score per tertile of DDE level in the full study population, controlling for age, sex, race/ethnicity, education, and APOE genotype and accounting for location. Confounders were selected on the basis of biological plausibility and 10% change in effect estimate. For samples with nondetectable levels of DDE (n=46), we imputed a value equal to half the limit of detection (0.075 ng/mL), as described by Lubin and colleagues8 and corrected for cholesterol levels. Regression analysis including the nondetects as zero value did not significantly change the OR estimate. To explore whether the presence of an ε4 allele of APOE modified the association between DDE levels and MMSE scores, we either stratified the data by genotype or used an interaction model (DDE*APOE4) with generalized estimating equations.

Baseline characteristics of the cohort are shown in Table 1. There were a total of 165 samples representing 79 control and 86 AD cases. The cohort comprised 94 women and 71 men, with women comprising 60% of the control and 55% of the AD cases. The presence of at least 1 APOE4 allele was found in 35% of control and 65% of AD cases.

Table Graphic Jump LocationTable 1.  Description of the Study Population

Dichlorodiphenyldichloroethylene (DDE) was detected in 70% of control and 80% of AD cases (Table 1), with mean levels 3.8-fold higher in the serum of AD cases (mean [SEM], 2.64 [0.35] ng/mg cholesterol) compared with control participants (mean [SEM], 0.69 [0.1] ng/mg cholesterol; P < .001; Figure 1). No other organochlorine pesticide besides DDE was found to be elevated in AD samples compared with control participants (data not shown). The association between serum DDE levels and AD is presented in Table 2. Levels of DDE were divided into tertiles, with the nondetects designated at half the limit of detection, and the OR was estimated using generalized estimating equations and corrected for age, sex, race/ethnicity, and location. Compared with the first tertile, the OR for AD diagnosis in the third tertile of DDE level was significantly increased (OR, 4.18; 95% CI, 2.54-5.82; P < .0001). The presence of an APOE ε4 allele alone was associated with increased AD diagnosis (OR, 3.70; 95% CI, 2.97-4.60; P < .0001). However, adjustment for APOE genotype did not significantly alter the association between DDE levels and AD diagnosis (Table 2). Furthermore, DDE levels did not differ based on APOE genotype (data not shown). To explore the potential influence of nondetects of DDE on AD diagnosis, we performed a sensitivity analysis by comparing the highest tertile of DDE against the nondetects and comparing the highest tertile against the lowest tertile when nondetects were excluded. Similar ORs to our original analysis were observed (eTable 1 in Supplement). Likewise, similar ORs were observed when the nondetects were assigned a value of zero (OR, 3.60; 95% CI, 1.23-10.57; P < .001).

Place holder to copy figure label and caption
Figure 1.
Serum Levels of Dichlorodiphenyldichloroethylene (DDE)

Serum levels of DDE are elevated in Alzheimer disease (AD). Data were pooled from University of Texas Southwestern Medical Center and Emory University. Levels of DDE are significantly higher in patients with AD (mean [SEM], 2.64 [0.35]) vs control participants (mean [SEM], 0.69 [0.10]; P < .001).

Graphic Jump Location
Table Graphic Jump LocationTable 2.  Odds of AD per Tertile of DDE Distribution

Mini-Mental State Examination scores were significantly lower in the highest DDE tertile (−0.841; 95% CI, −1.604 to −0.079; P = .03) (Table 3). Sensitivity analysis demonstrated that excluding nondetects (−1.605; 95% CI, −3.095 to −0.114; P = .04) or designating nondetects as zero (−2.628; 95% CI, −5.363 to 0.107; P = .06) resulted in similar effect estimates (eTable 2 in Supplement). There was a significant interaction between APOE status and DDE levels, where the MMSE score in those with an ε4 allele and DDE levels in the third tertile (OR, −1.6995; 95% CI, −3.293 to −0.106; P = .04) was significantly lower (P interaction = .04) compared with those without an ε4 allele (OR, −0.5272; 95% CI, −0.623 to −0.432); P < .0001). Serum DDE levels did not differ by genotype, suggesting this is a functional interaction.

Table Graphic Jump LocationTable 3.  APOE4 Polymorphism Modifies the Association Between DDE and MMSE Scoresa

To determine the relationship between serum and brain levels of DDE, we measured DDE in 11 matched brain and serum samples from patients with AD collected from the Washington University Alzheimer’s Disease Center. For these samples, the serum samples were taken within a year before death and collection of brain tissue. Mean (SEM) serum levels of DDE were 2.69 (0.75) ng/mg cholesterol and were not significantly different from and highly correlated with mean (SEM) brain levels (1.89 [0.46] ng/mg cholesterol; ρ = 0.95; eFigure in Supplement).

Finally, we sought to determine whether DDE, or its parent compound DDT, has a mechanistic association with AD. A recent study reported that altered network activity in a transgenic AD model, the APP overexpressing mouse, was associated with altered sodium channels.9 Because sodium channels are the molecular target of DDT and APP overexpression is a causative factor in AD,10 we hypothesized that DDT or DDE would increase APP levels. To determine this, we exposed cultured neuronal cells and measured the levels of APP, whose genetic overexpression is a risk factor for AD. Exposure of differentiated SY5Y cells to 1-μM DDE or DDT for 48 hours significantly increased APP levels by almost 50% (Figure 2).

Place holder to copy figure label and caption
Figure 2.
Dichlorodiphenyltrichloroethane (DDT) and Dichlorodiphenyldichloroethylene (DDE) Increase Levels of Amyloid Precursor Protein (APP) in Differentiated SH-SY5Y Cells

SY5Y cells were differentiated with retinoic acid and exposed to 1-μM DDT or DDE for 48 hours. Cells were fixed and stained with antimicrotubule-associated protein–2 (MAP-2) and anti-APP. Original magnification ×20.

Graphic Jump Location

Few studies to date have explored the potential for environmental exposures to contribute to AD. Here, we demonstrated that serum levels of DDE, the metabolite of the organochlorine pesticide DDT, are associated with AD diagnosis and AD severity, as assessed by MMSE. Furthermore, serum DDE levels in the third tertile and the presence of an APOE ε4 allele resulted in even greater cognitive impairment. Finally, we demonstrated that concentrations of DDE and its parent compound DDT, similar to those observed in highly exposed individuals in the general population of the United States,11,12 increased APP levels in cultured neuronal cells. Together, these data identify DDT/DDE exposure as an environmental risk factor for AD.

Dichlorodiphenyltrichloroethane (DDT) was extensively used from the 1940s through 1972 in the United States both in agriculture as a broad-spectrum insecticide and for control of vector-borne diseases including malaria. Although DDT undoubtedly led to major public health victories, the Environmental Protection Agency banned the use of DDT in the United States in 1972 because of concerns regarding its environmental persistence and potential effects on wildlife. At its peak in 1962, production of DDT in the United States was approximately 82 million kg.13 Currently, several countries around the world continue to use DDT legally and illegally for agricultural purposes, and it is an ingredient in the pesticide Dicofol. Although controversial, the World Health Organization14,15 supported reintroduction of DDT for malaria eradication in 2006. Thus, there is still significant exposure of human populations.

Levels of DDT and DDE have decreased significantly in the environment over the past 3 decades in the United States. However, DDE is still found in 75% to 80% of serum samples from the Centers for Disease Control and Prevention’s cross-sectional National Health and Nutrition Examination Survey.16 This is likely the result of the exceptionally long half-life of DDE (approximately 8-10 years) and continuing exposure from the import of food from countries where DDT is still used or from legacy contamination of soil and waterways in the United States.17 The serum DDE concentrations reported here are consistent with those reported by the National Health and Nutrition Examination Survey, with the highest levels in the same range as observed in the 95th percentile.16 Serum concentrations of DDE are much higher elsewhere in the world where DDT was phased out later or is still used such as Spain and India.18,19 Importantly, we also found that serum levels are highly correlated with brain levels, which has not been reported before, but is consistent with the high correlation between serum and adipose tissue.20 Thus, serum levels appear to be an accurate surrogate for DDE levels in the brain.

Although DDT exerts its insecticidal activity through disruption of the nervous system, neither DDT nor DDE are particularly toxic (rat oral LD50s = 113 and 880 mg/kg, respectively).21 Indeed, administration of DDT or DDE to human individuals for up to 18 months did not cause overt toxicity.22,23 However, chronic exposure to DDT and DDE has raised concerns about a variety of potential adverse health effects.13,14,24 Unfortunately, to our knowledge, there are few human studies that have explored the potential neurotoxicity of DDT/DDE. Cueto and colleagues23 exposed volunteers to 3.5 or 35 mg DDT per day for 12 to 18 months and observed no effects on neurological function. However, 2 other studies found that workers engaged in spraying DDT displayed cognitive dysfunction, although no measurements of DDT or DDE were available for either study.25,26 Likewise, a large community-based study identified that occupational exposure to organochlorine pesticides was associated with dementia and AD.5 One small study reported that DDT was found more often in AD brains (n = 7) compared with control participants (n = 14).27 Recently, we found an association of serum DDE levels with a diagnosis of AD in a small pilot study, and another study in India found higher serum levels of several organochlorine pesticides, including DDE, in patients with AD.6,28 Taken together with these studies, our data provide strong support for a role of DDT/DDE in AD. However, we questioned whether this association was mechanistically plausible.

Treatment of SH-SY5Y cells with concentrations of DDT and DDE in the range of concentrations observed in the serum of humans administered 5 to 20 mg of DDT or DDE for 2 to 6 months,22 in people from an Alabama community with high levels of DDT exposure from industrial dumping of DDT11 and in people residing in a community near a Superfund site in Maryland,12 resulted in increased levels of APP, suggesting a possible mechanism for our epidemiological finding.

Our study had a number of strengths including the largest sample size to date for this type of study and the use of well-characterized clinical populations from 2 different geographical locations. The sensitivity analysis ensures that the results were not driven by differences in sampling site or differences in nondetects between cases and control participants. The serum levels of DDE are consistent with those most recently reported by the National Health and Nutrition Examination Survey, suggesting that the cases and control participants were representative of the general population of the United States.2 Additionally, we provided data demonstrating that serum levels of DDE are highly correlated with brain levels.

There were also limitations to our study. As with our previous study and one other that measured pesticides in the serum of patients with AD, we were limited to studying the persistent organochlorine pesticides.6,28 Thus, the possibility that other nonpersistent pesticides, such as organophosphates,5 may contribute to the development of AD in our cohorts cannot be ruled out. A recent study from India found that in addition to DDE, dieldrin and β-hexachlorocyclohexane were elevated in serum samples from patients with AD.28 However, no detectable levels of dieldrin were found in this study or in more than 200 human serum samples we analyzed in previous studies,6,7 and β-hexachlorocyclohexane levels significantly decreased in the United States and were not associated with AD in this study.7 Some patients with AD in our cohort (17 of 86) had nondetectable levels of DDE and control participants were present in the top tertile of DDE levels. This suggests that exposure to DDE may contribute to AD only in a subset of cases, perhaps those with genetic polymorphisms that render them more susceptible to DDT/DDE exposure.

Our findings support epidemiological studies reporting an association of AD with occupational exposure to organochlorine pesticides5,28,29 and extend them by identifying DDT/DDE as a specific organochlorine pesticide linked to AD in a clinical population from the United States. Indeed, the OR for the association of elevated serum DDE levels with AD is as high as that for APOE and the recently reported TREM2.3,30,31 Because elevated DDE levels were associated with significantly worse MMSE performance and exacerbated by the presence of an APOE ε4 allele, measurement of serum DDE levels accompanied by APOE genotyping might be a useful clinical measure to identify individuals who may be at increased risk for AD. The finding that DDT and DDE increase APP levels in cells provides a mechanistic plausibility to the association between these exposures and AD. If elevation of APP by DDT and/or DDE is confirmed in animal studies and humans, it may provide an avenue for a targeted treatment of individuals with high levels of DDE, such as beta-site APP-cleaving enzyme inhibitors, to prevent cleavage of elevated APP to amyloid-β 42.

Corresponding Author: Jason R. Richardson, PhD, Department of Environmental and Occupational Medicine, Rutgers Robert Wood Johnson Medical School and Environmental and Occupational Health Sciences Institute, 170 Frelinghuysen Road, EOHSI 340, Piscataway, NJ 08854 (jricha3@eohsi.rutgers.edu).

Accepted for Publication: December 4, 2013.

Published Online: January 27, 2014. doi:10.1001/jamaneurol.2013.6030.

Author Contributions: Dr Richardson had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Study concept and design: Richardson, Levey, German.

Acquisition of data: Richardson, von Stein, Hossain, Buckley, Gearing, Levey, German.

Analysis and interpretation of data: Richardson, Roy, Shalat, Levey, German.

Drafting of the manuscript: Richardson, Roy, Buckley, German.

Critical revision of the manuscript for important intellectual content: Richardson, Roy, Shalat, von Stein, Hossain, Gearing, Levey, German.

Statistical analysis: Richardson, Roy, Shalat.

Obtained funding: Richardson, Levey, German.

Administrative, technical, and material support: von Stein, Hossain, Buckley, Gearing, Levey.

Study supervision: Richardson.

Conflict of Interest Disclosures: None reported.

Funding/Support: This research was supported in part by National Institutes of Health grants P30ES005022, T32ES07148, R21NS072097, R01ES015991, P30AG012300, P50AG05681, and P50AG025688.

Role of the Sponsor: The National Institutes of Health had no role in the design and conduct of the study; collection, management, analysis, or interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.

Brookmeyer  R, Johnson  E, Ziegler-Graham  K, Arrighi  HM.  Forecasting the global burden of Alzheimer's disease. Alzheimers Dement. 2007;3(3):186-191.
PubMed   |  Link to Article
Pedersen  NL.  Reaching the limits of genome-wide significance in Alzheimer disease: back to the environment. JAMA. 2010;303(18):1864-1865.
PubMed   |  Link to Article
Schellenberg  GD, Montine  TJ.  The genetics and neuropathology of Alzheimer’s disease. Acta Neuropathol. 2012;124(3):305-323.
PubMed   |  Link to Article
Santibáñez  M, Bolumar  F, García  AM.  Occupational risk factors in Alzheimer’s disease: a review assessing the quality of published epidemiological studies. Occup Environ Med. 2007;64(11):723-732.
PubMed   |  Link to Article
Hayden  KM, Norton  MC, Darcey  D,  et al; Cache County Study Investigators.  Occupational exposure to pesticides increases the risk of incident AD: the Cache County Study. Neurology. 2010;74(19):1524-1530.
PubMed   |  Link to Article
Richardson  JR, Shalat  SL, Buckley  B,  et al.  Elevated serum pesticide levels and risk of Parkinson disease. Arch Neurol. 2009;66(7):870-875.
PubMed
Richardson  JR, Roy  A, Shalat  SL,  et al.  β-Hexachlorocyclohexane levels in serum and risk of Parkinson’s disease. Neurotoxicology. 2011;32(5):640-645.
PubMed   |  Link to Article
Lubin  JH, Colt  JS, Camann  D,  et al.  Epidemiologic evaluation of measurement data in the presence of detection limits. Environ Health Perspect. 2004;112(17):1691-1696.
PubMed   |  Link to Article
Verret  L, Mann  EO, Hang  GB,  et al.  Inhibitory interneuron deficit links altered network activity and cognitive dysfunction in Alzheimer model. Cell. 2012;149(3):708-721.
PubMed   |  Link to Article
Goate  A, Hardy  J.  Twenty years of Alzheimer’s disease-causing mutations. J Neurochem. 2012;120(suppl 1):3-8.
PubMed   |  Link to Article
Kreiss  K, Zack  MM, Kimbrough  RD, Needham  LL, Smrek  AL, Jones  BT.  Cross-sectional study of a community with exceptional exposure to DDT. JAMA. 1981;245(19):1926-1930.
PubMed   |  Link to Article
Gaffney  SH, Curriero  FC, Strickland  PT, Glass  GE, Helzlsouer  KJ, Breysse  PN.  Influence of geographic location in modeling blood pesticide levels in a community surrounding a US Environmental Protection Agency superfund site. Environ Health Perspect. 2005;113(12):1712-1716.
PubMed   |  Link to Article
Agency for Toxic Substances and Disease Registry. Toxicological Profile for DDT, DDE, DDD. Atlanta, GA: US Department of Health and Human Services, Public Health Service; 2002.
Eskenazi  B, Chevrier  J, Rosas  LG,  et al.  The Pine River statement: human health consequences of DDT use. Environ Health Perspect. 2009;117(9):1359-1367.
PubMed   |  Link to Article
Rehwagen  C.  WHO recommends DDT to control malaria. BMJ. 2006;333(7569):622.
PubMed   |  Link to Article
Centers for Disease Control and Prevention. Fourth National Report on Human Exposure to Environmental Chemicals. 2009. http://www.cdc.gov/exposurereport/pdf/FourthReport.pdf. Accessed May 31, 2013.
Food and Drug Administration. Pesticide Monitoring Program 2008. http://www.fda.gov/Food/FoodborneIllnessContaminants/Pesticides/ucm228867.htm. Accessed May 31, 2013.
Botella  B, Crespo  J, Rivas  A, Cerrillo  I, Olea-Serrano  MF, Olea  N.  Exposure of women to organochlorine pesticides in Southern Spain. Environ Res. 2004;96(1):34-40.
PubMed   |  Link to Article
Bhatnagar  VK, Kashyap  R, Zaidi  SS, Kulkarni  PK, Saiyed  HN.  Levels of DDT, HCH, and HCB residues in human blood in Ahmedabad, India. Bull Environ Contam Toxicol. 2004;72(2):261-265.
PubMed   |  Link to Article
Rusiecki  JA, Matthews  A, Sturgeon  S,  et al.  A correlation study of organochlorine levels in serum, breast adipose tissue, and gluteal adipose tissue among breast cancer cases in India. Cancer Epidemiol Biomarkers Prev. 2005;14(5):1113-1124.
PubMed   |  Link to Article
Gaines  TB.  Acute toxicity of pesticides. Toxicol Appl Pharmacol. 1969;14(3):515-534.
PubMed   |  Link to Article
Morgan  DP, Roan  CC.  Absorption, storage, and metabolic conversion of ingested DDT and DDT metabolites in man. Arch Environ Health. 1971;22(3):301-308.
PubMed   |  Link to Article
Cueto  C  Jr, Durham  WF, Hayes  WJ  Jr.  The effect of known repeated oral doses of chlorophenothane (DDT) in man. J Am Med Assoc. 1956;162(9):890-897.
PubMed   |  Link to Article
Rogan  WJ, Chen  A.  Health risks and benefits of bis(4-chlorophenyl)-1,1,1-trichloroethane (DDT). Lancet. 2005;366(9487):763-773.
PubMed   |  Link to Article
Misra  UK, Nag  D, Murti  CR.  A study of cognitive functions in DDT sprayers. Ind Health. 1984;22(3):199-206.
PubMed   |  Link to Article
van Wendel de Joode  B, Wesseling  C, Kromhout  H, Monge  P, García  M, Mergler  D.  Chronic nervous-system effects of long-term occupational exposure to DDT. Lancet. 2001;357(9261):1014-1016.
PubMed   |  Link to Article
Fleming  L, Mann  JB, Bean  J, Briggle  T, Sanchez-Ramos  JR.  Parkinson’s disease and brain levels of organochlorine pesticides. Ann Neurol. 1994;36(1):100-103.
PubMed   |  Link to Article
Singh  N, Chhillar  N, Banerjee  B, Bala  K, Basu  M, Mustafa  M.  Organochlorine pesticide levels and risk of Alzheimer’s disease in north Indian population. Hum Exp Toxicol. 2013;32(1):24-30.
PubMed   |  Link to Article
Baldi  I, Lebailly  P, Mohammed-Brahim  B, Letenneur  L, Dartigues  JF, Brochard  P.  Neurodegenerative diseases and exposure to pesticides in the elderly. Am J Epidemiol. 2003;157(5):409-414.
PubMed   |  Link to Article
Guerreiro  R, Wojtas  A, Bras  J,  et al; Alzheimer Genetic Analysis Group.  TREM2 variants in Alzheimer’s disease. N Engl J Med. 2013;368(2):117-127.
PubMed   |  Link to Article
Jonsson  T, Stefansson  H, Steinberg  S,  et al.  Variant of TREM2 associated with the risk of Alzheimer’s disease. N Engl J Med. 2013;368(2):107-116.
PubMed   |  Link to Article

Figures

Place holder to copy figure label and caption
Figure 1.
Serum Levels of Dichlorodiphenyldichloroethylene (DDE)

Serum levels of DDE are elevated in Alzheimer disease (AD). Data were pooled from University of Texas Southwestern Medical Center and Emory University. Levels of DDE are significantly higher in patients with AD (mean [SEM], 2.64 [0.35]) vs control participants (mean [SEM], 0.69 [0.10]; P < .001).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.
Dichlorodiphenyltrichloroethane (DDT) and Dichlorodiphenyldichloroethylene (DDE) Increase Levels of Amyloid Precursor Protein (APP) in Differentiated SH-SY5Y Cells

SY5Y cells were differentiated with retinoic acid and exposed to 1-μM DDT or DDE for 48 hours. Cells were fixed and stained with antimicrotubule-associated protein–2 (MAP-2) and anti-APP. Original magnification ×20.

Graphic Jump Location

Tables

Table Graphic Jump LocationTable 1.  Description of the Study Population
Table Graphic Jump LocationTable 2.  Odds of AD per Tertile of DDE Distribution
Table Graphic Jump LocationTable 3.  APOE4 Polymorphism Modifies the Association Between DDE and MMSE Scoresa

References

Brookmeyer  R, Johnson  E, Ziegler-Graham  K, Arrighi  HM.  Forecasting the global burden of Alzheimer's disease. Alzheimers Dement. 2007;3(3):186-191.
PubMed   |  Link to Article
Pedersen  NL.  Reaching the limits of genome-wide significance in Alzheimer disease: back to the environment. JAMA. 2010;303(18):1864-1865.
PubMed   |  Link to Article
Schellenberg  GD, Montine  TJ.  The genetics and neuropathology of Alzheimer’s disease. Acta Neuropathol. 2012;124(3):305-323.
PubMed   |  Link to Article
Santibáñez  M, Bolumar  F, García  AM.  Occupational risk factors in Alzheimer’s disease: a review assessing the quality of published epidemiological studies. Occup Environ Med. 2007;64(11):723-732.
PubMed   |  Link to Article
Hayden  KM, Norton  MC, Darcey  D,  et al; Cache County Study Investigators.  Occupational exposure to pesticides increases the risk of incident AD: the Cache County Study. Neurology. 2010;74(19):1524-1530.
PubMed   |  Link to Article
Richardson  JR, Shalat  SL, Buckley  B,  et al.  Elevated serum pesticide levels and risk of Parkinson disease. Arch Neurol. 2009;66(7):870-875.
PubMed
Richardson  JR, Roy  A, Shalat  SL,  et al.  β-Hexachlorocyclohexane levels in serum and risk of Parkinson’s disease. Neurotoxicology. 2011;32(5):640-645.
PubMed   |  Link to Article
Lubin  JH, Colt  JS, Camann  D,  et al.  Epidemiologic evaluation of measurement data in the presence of detection limits. Environ Health Perspect. 2004;112(17):1691-1696.
PubMed   |  Link to Article
Verret  L, Mann  EO, Hang  GB,  et al.  Inhibitory interneuron deficit links altered network activity and cognitive dysfunction in Alzheimer model. Cell. 2012;149(3):708-721.
PubMed   |  Link to Article
Goate  A, Hardy  J.  Twenty years of Alzheimer’s disease-causing mutations. J Neurochem. 2012;120(suppl 1):3-8.
PubMed   |  Link to Article
Kreiss  K, Zack  MM, Kimbrough  RD, Needham  LL, Smrek  AL, Jones  BT.  Cross-sectional study of a community with exceptional exposure to DDT. JAMA. 1981;245(19):1926-1930.
PubMed   |  Link to Article
Gaffney  SH, Curriero  FC, Strickland  PT, Glass  GE, Helzlsouer  KJ, Breysse  PN.  Influence of geographic location in modeling blood pesticide levels in a community surrounding a US Environmental Protection Agency superfund site. Environ Health Perspect. 2005;113(12):1712-1716.
PubMed   |  Link to Article
Agency for Toxic Substances and Disease Registry. Toxicological Profile for DDT, DDE, DDD. Atlanta, GA: US Department of Health and Human Services, Public Health Service; 2002.
Eskenazi  B, Chevrier  J, Rosas  LG,  et al.  The Pine River statement: human health consequences of DDT use. Environ Health Perspect. 2009;117(9):1359-1367.
PubMed   |  Link to Article
Rehwagen  C.  WHO recommends DDT to control malaria. BMJ. 2006;333(7569):622.
PubMed   |  Link to Article
Centers for Disease Control and Prevention. Fourth National Report on Human Exposure to Environmental Chemicals. 2009. http://www.cdc.gov/exposurereport/pdf/FourthReport.pdf. Accessed May 31, 2013.
Food and Drug Administration. Pesticide Monitoring Program 2008. http://www.fda.gov/Food/FoodborneIllnessContaminants/Pesticides/ucm228867.htm. Accessed May 31, 2013.
Botella  B, Crespo  J, Rivas  A, Cerrillo  I, Olea-Serrano  MF, Olea  N.  Exposure of women to organochlorine pesticides in Southern Spain. Environ Res. 2004;96(1):34-40.
PubMed   |  Link to Article
Bhatnagar  VK, Kashyap  R, Zaidi  SS, Kulkarni  PK, Saiyed  HN.  Levels of DDT, HCH, and HCB residues in human blood in Ahmedabad, India. Bull Environ Contam Toxicol. 2004;72(2):261-265.
PubMed   |  Link to Article
Rusiecki  JA, Matthews  A, Sturgeon  S,  et al.  A correlation study of organochlorine levels in serum, breast adipose tissue, and gluteal adipose tissue among breast cancer cases in India. Cancer Epidemiol Biomarkers Prev. 2005;14(5):1113-1124.
PubMed   |  Link to Article
Gaines  TB.  Acute toxicity of pesticides. Toxicol Appl Pharmacol. 1969;14(3):515-534.
PubMed   |  Link to Article
Morgan  DP, Roan  CC.  Absorption, storage, and metabolic conversion of ingested DDT and DDT metabolites in man. Arch Environ Health. 1971;22(3):301-308.
PubMed   |  Link to Article
Cueto  C  Jr, Durham  WF, Hayes  WJ  Jr.  The effect of known repeated oral doses of chlorophenothane (DDT) in man. J Am Med Assoc. 1956;162(9):890-897.
PubMed   |  Link to Article
Rogan  WJ, Chen  A.  Health risks and benefits of bis(4-chlorophenyl)-1,1,1-trichloroethane (DDT). Lancet. 2005;366(9487):763-773.
PubMed   |  Link to Article
Misra  UK, Nag  D, Murti  CR.  A study of cognitive functions in DDT sprayers. Ind Health. 1984;22(3):199-206.
PubMed   |  Link to Article
van Wendel de Joode  B, Wesseling  C, Kromhout  H, Monge  P, García  M, Mergler  D.  Chronic nervous-system effects of long-term occupational exposure to DDT. Lancet. 2001;357(9261):1014-1016.
PubMed   |  Link to Article
Fleming  L, Mann  JB, Bean  J, Briggle  T, Sanchez-Ramos  JR.  Parkinson’s disease and brain levels of organochlorine pesticides. Ann Neurol. 1994;36(1):100-103.
PubMed   |  Link to Article
Singh  N, Chhillar  N, Banerjee  B, Bala  K, Basu  M, Mustafa  M.  Organochlorine pesticide levels and risk of Alzheimer’s disease in north Indian population. Hum Exp Toxicol. 2013;32(1):24-30.
PubMed   |  Link to Article
Baldi  I, Lebailly  P, Mohammed-Brahim  B, Letenneur  L, Dartigues  JF, Brochard  P.  Neurodegenerative diseases and exposure to pesticides in the elderly. Am J Epidemiol. 2003;157(5):409-414.
PubMed   |  Link to Article
Guerreiro  R, Wojtas  A, Bras  J,  et al; Alzheimer Genetic Analysis Group.  TREM2 variants in Alzheimer’s disease. N Engl J Med. 2013;368(2):117-127.
PubMed   |  Link to Article
Jonsson  T, Stefansson  H, Steinberg  S,  et al.  Variant of TREM2 associated with the risk of Alzheimer’s disease. N Engl J Med. 2013;368(2):107-116.
PubMed   |  Link to Article

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eFigure. Correlation Analysis of Brain and Serum DDE Levels

eTable 1. Effect of DDE Levels on AD (Sensitivity Analysis Including Nondetects)

eTable 2. Effect of DDE Levels on MMSE Scores (Sensitivity Analysis)

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